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Updated: Sep 06, 2026
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ASCP ASCP-MLT Exam Overview:
| Certification Vendor: | American Society for Clinical Pathology Board of Certification (ASCP BOC) |
|---|---|
| Exam Name: | Medical Laboratory Technician |
| Exam Number: | MLT(ASCP) |
| Real Exam Qty: | Approximately 100 |
| Available Languages: | English |
| Exam Format: | Multiple-choice questions (one best answer), Computer Adaptive Testing (CAT) |
| Exam Price: | Application fee (USD 235–250, varies by region and international discounts) |
| Certificate Validity Period: | Certification maintenance required (completion of ongoing continuing education every 3 years) |
| Exam Duration: | 150 minutes |
| Related Certifications: | MLS(ASCP) Medical Laboratory Scientist PBT(ASCP) Phlebotomy Technician |
| Passing Score: | Scaled score of ≥ 400 (on 100–999 scale, CAT scoring) |
| Sample Questions: | ASCP ASCP-MLT Sample Questions |
| Exam Way: | Computer-based testing delivered at Pearson VUE test centres |
| Pre Condition: | Completion of accredited MLT program or equivalent education/training OR relevant clinical experience following ASCP BOC eligibility routes |
| Official Syllabus URL: | https://www.ascp.org/boc/explore-credentials/view-all-credentials/MLT |
ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Urinalysis and Other Body Fluids | 5–10% | - Physical and chemical testing - Microscopic analysis and disease correlation |
| Topic 2: Laboratory Operations | 5–10% | - Instrumentation and laboratory mathematics - Quality assessment and safety |
| Topic 3: Hematology | 20–25% | - Laboratory testing and hemostasis - Physiology and disease states |
| Topic 4: Chemistry | 20–25% | - Electrolytes, acid-base, and toxicology - Carbohydrates, lipids, and enzymes |
| Topic 5: Immunology | 5–10% | - Principles of immunology - Serologic procedures and immune diseases |
| Topic 6: Blood Banking | 15–20% | - Transfusion practice and testing - Blood group systems and immunology - Blood products and components |
| Topic 7: Microbiology | 15–20% | - Bacteriology and mycology procedures - Virology and parasitology testing |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
Question 1
The creatinine clearance for this patient is 100 mL/min.
Creatinine clearance values are calculated using the following equation:
Creatinine Clearance (mL / min) = (Urine Creatinine / Serum Creatinine) x Urine Volume (mL) / [ time (hr) x
60 ]
For this patient Creatinine clearance (mL/min)= (120/1.5) x (1800 / [24 x 60])
80 x 1800/1440, which is 80 x 1.25, or 100 mL/min
A 45-year-old male of average height and weight had a serum creatinine of 1.5 mg/dL and urine creatinine was
120 mg/dL; the total volume of urine collected over a 24-hour period was 1,800 mL. Calculate the creatinine clearance for this patient in mL/min.
A. 100
B. 144
C. 56
D. 177
E. 225
Question 2
Spirochetes such as Borrelia and Treponemes are best visualized using darkfield microscopy. Borrelia, which causes Relapsing fever, can be visualized under darkfield microscopy of wet preps of peripheral blood.
Treponema can be visualized by darkfield microscopy of early primary lesions, and aspirates of affected lymph nodes.
Which of the following organisms is best visualized by use of a darkfield microscope:
A. Borrelia
B. Campybacter
C. Streptococcu
D. Entamoeba
Question 3
A blood culture to evaluate septicemia is performed in:
A. hematology.
B. toxicology.
C. urinalysis.
D. microbiology.
Question 4
Which of the following agencies administers the certification test that you will be eligible to take at the end of your 4 semester?
A. ASCP
B. ASPT
C. AAMA
D. AARP
Question 5
Heparin is the additive of choice for blood gas syringe collection as it has the least amount of interference with the blood gas analytes measured.
Which of the following additives should be used for the collection of a sample for blood gas analysis:
A. Heparin
B. Sodium citrate
C. Sodium oxalate
D. EDTA
Solutions:
| Question 1 Answer: A | Question 2 Answer: A | Question 3 Answer: D | Question 4 Answer: A | Question 5 Answer: A |
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