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ASCP ASCP-MLT Exam Syllabus Topics:

SectionWeightObjectives
Microbiology15-20%- Specimen collection and culture techniques
- Mycology, parasitology, and virology
- Bacteriology and antimicrobial susceptibility
- Identification of pathogenic microorganisms
Clinical Chemistry20-25%- Endocrinology and therapeutic drug monitoring
- Electrolytes, acid-base balance, and blood gases
- Carbohydrates, lipids, proteins, and enzymes
- Toxicology and liver/kidney function tests
Hematology20-25%- White blood cell morphology and diseases
- Hemostasis and coagulation testing
- Routine and automated hematology procedures
- Red blood cell physiology and disorders
Blood Banking (Immunohematology)15-20%- Blood components and storage
- Compatibility testing
- Blood group systems and antigens
- Transfusion reactions and adverse events
- Antibody detection and identification
Immunology and Serology5-10%- Immune system principles and components
- Immunologic testing methodologies
- Infectious disease serology
- Autoimmune and immunodeficiency disorders
Laboratory Operations5-10%- Quality control and quality assurance
- Laboratory mathematics and instrumentation
- Pre-analytical, analytical, and post-analytical processes
- Laboratory safety and regulatory compliance
Urinalysis and Other Body Fluids5-10%- Urine formation and composition
- Semen and amniotic fluid testing
- Physical, chemical, and microscopic analysis
- Cerebrospinal, serous, and synovial fluids

ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. Which of the following agencies administers the certification test that you will be eligible to take at the end of your 4 semester?

A) ASCP
B) ASPT
C) AAMA
D) AARP


2. This Gram stain is under-decolorized
This microscopic field is representative of other fields that were observed on a Gram-stained smear. Which of the following describes the quality of the smear?

A) Over-decolorized
B) Under-decolorized
C) Sufficiently decolorized


3. Protein in urine can be confirmed using sulfosalicylic acid (SSA) precipitation. The SSA reagent is added to a small volume of urine. Acidification causes precipitation of protein in the sample, which is subjectively graded as trace, 1+, 2+, 3+ or 4+. SSA reaction will detect albumin, globulins, and Bence-Jones proteins.
Which of the following would be the most appropriate method to confirm a positive protein from a urine dipstick:

A) Sulfosalicylic acid precipitation
B) Immunoelectropheresis
C) Heat precipitation
D) Protein electrophoresis


4. The DAT, or direct antiglobulin test, is used to detect antibodies coating RBC's in vivo.
The Direct Antiglobulin Test (DAT) is usually used to detect:

A) Antibody-hapten reactions
B) Antigens in the plasma
C) Antibodies coating red cells
D) Antibodies in the plasma
E) Antigens coating red cells


5. The negative antigen frequencies are used in the formula below to determine the percent of type-specific units that would be compatible for the patient. Negative antigen frequencies are determined by subtracting the percent antigen frequency from 100%. The negative antigen frequency for K in this case is .91 (1.00 - .09) and the negative antigen frequency for Fya is .34 (1.00 - .66).
% compatible units available = 100 x (Neg frequency #1 x Neg frequency #2...) For this case, the calculation is:
% compatible units available = 100 x (0.91 x 0.34) = 30.94 or 31%
If the antigen frequencies for K = 0.09 and Fya = 0.66, what percent of type-specific units would be compatible for a patient with anti-K and anti-Fya?

A) 31
B) 100
C) 10
D) 43
E) 92


Solutions:

Question # 1
Answer: A
Question # 2
Answer: B
Question # 3
Answer: A
Question # 4
Answer: C
Question # 5
Answer: A

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